5 µL anti-cAMP-Cryptate. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. 315 can't find zero order. The calculated LOQ values were 0. Winnersh Triangle. SpectraMax ® iD3 and iD5 Multi-Mode . SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. TNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. 2 in. 08, 0. SoftMax Pro GxP Compliance Software. Detection of pro-inflammatory factors. Get a front row view of your cells. Research Article Streptococcus lutetiensis Induces Autophagy via Oxidative Stress in Bovine Mammary Epithelial Cells Peng Chen ,1 Jingyue Yang,1 Naiwen Wu,1 Bo Han,1 John P. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Next, an AlphaLISA assay was developed to detect levels of pro-inflammatory factors produced by endothelial cells in response to TNF-alpha stimulation. We would like to show you a description here but the site won’t allow us. 99 to $1999. Prismatic colour and motion for everyone. Download PDF. The treatments were. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your intial investment. 12911151) 0. Description 1. Thetypeof luciferase(Renilla. D. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. 0. 8 Antibodies and. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any application or budget. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. SPECTRAmax 190 Microplate Spectrophotometer Operator™s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax 190 control panel are shown in boxed Helvetica type. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. SpectraMax i3x Multi-Mode Microplate Reader SystemSpectraMax® i3x dari Molecular Devices adalah sistem deteksi berbasis multi-mode yang berevolusi menyesuaik. This instrument also provides the option of modular upgrades for Western Blotting,. china@moldev. Multi-Mode Reader. Molecular Devicesの全商品を見る. Enzyme activity was presented as absolute activity in a unit of nmol g −1 dry soil h −1. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. 2 (Molecular Devices, San Jose, CA, USA). Quickly and easily expand the capabilities of your SpectraMax Paradigm or i3x Multi-Mode Microplate reader to include Tunable Wavelength detection using the. , 2011). The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. ; The top 3 importers of Spectramax are India with 365. This is not due to a temperature-dependent alteration in cellular autofluorescence,. • Removing the transport locks from the PMT shutters and drawers. UV-Vis absorbance, fluorescence and luminescence • Accommodates plate types from six to 384-well format • Easily upgradeable with interchangeable filter cubesSpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. 312 gain calibration failed. The drawing shows a standard microplate of 15 mm height covered with a lid. The SpectraMax ABS readers use a grating monochromator to select the exact wavelength needed for every assay. SpectraMax i3, i3x, and iD5 readers. i3. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataBased on the SpectraMax i3x system, ACROBiosystems provides biomedical research and development services for customers seeking quantification and detection of antibodies-antigen interaction. Prepare your i3/i3x instrument for shipment. Bookings are free, but you will need to use iLab to book. SpectraMax i3x 多功能微孔板读板机 SpectraMax Paradigm 多功能微孔板读板机 SpectraMax M5e 多功能微孔板读板机 扫一扫关注我 们的官方微信 Molecular Devices 大中华区 全国咨询服务热线: 400-820-3586 Email: info. 7°C (gradient heating design). Microplate Readers SpectraMax. Find regional support. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. Examples: fiPress carefully to engage,fl fiDo not press down. The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. Description 1. • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . Molecular Devices社の製品: 吸収度マルチモードマイクロプレートリーダー SpectraMax® i3x に関するすべての情報をご覧ください。. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. The SpectraMax MiniMax 300 Imaging Cytometer option is a field-upgradeable option for the SpectraMax i3/i3x Multi-Mode Detection Platform that offers cellular imaging capability. Example: [Drawer]. The user guide also covers the features, specifications, and software of the reader, as well as the installation and. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. Data Analysis. User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Image analysis provides accurate data. Dependingontheapplication,theinstrumentcanread6,12,24,48,96,and384-well microplates. 312 gain calibration failed. D 1 1. If you do not have an iLab account you must first make one: SpectraMax iD3 User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax iD3 Multi-Mode Microplate Reader, a versatile instrument that measures absorbance, fluorescence, and luminescence. Prime function 20 amol. Figure 1. 2. The SpectraMax i3x reader provides the With these results, as well as CV values below 6% for all standards, both the SpectraMax i3x and the SpectraMax iD5 readers demonstrate high sensitivity and a wide dynamic range for the IP-One assay. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellThe absorbance was recorded on a spectra Max i3x microplate reader (Molecular Devices, USA) at 450 nm. 5014038F 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 11 Applications 12 DualPhotomultiplierTubes 12，相关视频：多功能酶标仪SpectraMax i3x的维护操作-注射器卡盒，多功能酶标仪SpectraMax iD5的维护操作-注射器卡盒，多功能酶标仪SpectraMax i3x的维护操作-卡盒的安装和拆卸，细胞计数及活率分析仪 Vi-CELL BLU 功能&操作讲解，CRISPR基因编辑系统介绍：Cas9和Cas12a，流式. 0 mm hex key. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Itemploysa luciferaselabel(where“label”generallymeansfusedbytheuseofexpressionvectors)forthe oneproteinandaGreenFluorescentProtein(GFP)variantlabelfortheother. The intensity of the emitted light can be measured easily with the SpectraMax i3x reader. Figure 1. 3) At the bottom of the strip, click on "Show. SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. 3. Animal imaging assay was performed on a IVIS R Lumina Series III small animal optical system. com The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Transfected cells were imaged on the SpectraMax MiniMax 300 Imaging Cytometer. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. Amplitude : 1. SCGPU05RE)A SpectraMax ® i3x was used to determine uo-rescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. Molecular Devices, LLC Reading Modes: Endpoint and Kinetic; Optical System: Spectral Fusion Illumination. Sunnyvale, CA, USA) with excitation at 475 nm. SpectraMax i3x and Paradigm readers require the FP-FLUO detection cartridge. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strengthCapture flash assays with ease The SpectraMax i3x Injector Cartridge with SmartInject® Technology expands your research capabilities to include flash-based applications including dual luciferase and ATP assays. 99. Robust,. Spectranet customer portalSpectraMax i3x Multi-Mode Microplate Reader with MiniMax Imaging Cytometer by Molecular Devices® Manufacturer Molecular Devices® | Model: i3x | Available Worldwide 4. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. Figure 4. Find regional support. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence and luminescence. Get a front row view of your cells. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. SpectraMax i3x Multi-Mode Detection Platform User Guide. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. Spectranet customer portalSpectraMax i3/i3x Multi-Mode Microplate Reader SoftMax Pro Software 0 500 1000 1500 2000 2500 3000 0 1´ 106 2´ 106 3´ 106 dsDNA (pg/we l) R F U 02 4 6 8 1 0 2. 13. As. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email drugdiscoverytech@lifetech. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. SpectraMax M 시리즈 Microplate Reader, Cuvette Port, IQ OQ 검증 | Molecular Devices. The protocols are also available on the SoftMax Pro Software protocol sharing. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Bioz Stars score: 98/100, based on 1 PubMed citations. Multimode Readers Comparison Chart. It allows you to explore cellular pathways and protein activation and expression in one system. Read height should be optimized for . Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. The microplate was then placed in a SpectraMax i3x reader and incubated at 37°C for the duration of the kinetic measurement. The chemical reduction of resazurin was evaluated incubating each well of the microtiter plate with 10 μL (10% of final well volume) of 0. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. StainFree Cell Detection Algorithm. Published Dec 16, 2020 | Updated Dec 16, 2020. An MPO assay has been performed to quantify the inflammation reduced by the GAR-PLGA-ES100 NPs in the inflamed cells . Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. 4. 3: The cuvette chamber. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. ) deep, preferably with access on all sides. The information below applies to a standard 96 well plate, set to a linear shake mode. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. In this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. ) wide and 65 cm (25. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. It allows you to explore cellular pathways and protein activation and expression in one system. 200-800 nm using Spectra Max i3X microplate reader. The SpectraMax i3x reader provides theWith these results, as well as CV values below 6% for all standards, both the SpectraMax i3x and the SpectraMax iD5 readers demonstrate high sensitivity and a wide dynamic range for the IP-One assay. +44 118 944 8000. The Molecular Devices SpectraMax i3x Microplate Reader is a multi-mode microplate reader, able to measure UV/vis, fluorescence and luminescence. We would like to show you a description here but the site won’t allow us. infoboxeu@moldev. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. The absorbance at 645 nm was subsequently determined using a Spectra Max i3x microplate reader (Molecular Devices). SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataThe SpectraMax i3x reader and MiniMax cytometer can be used with FluoroBlok cell culture inserts to directly count cells from high-quality images to measure cellular migration over time. This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. Spectranet customer portal The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. china@moldev. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. When used in combination with the SpectraMax Injector Cartridge, this system enables a wealth of flash type luminescence applications including reporter gene and other assays with exceptional sensitivity and dynamic range. Increased fluorescence performance. 吸収度マイクロプレートリーダー SpectraMax® Mini. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,i3x Multi-Mode Microplate Reader. Explore a wealth of applications with the future-ready SpectraMax i3x. The SoftMax Pro Software quickly. 313 reference gain check fail. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. SpectraMax i3x Multi-Mode Detection Platform. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Superior flexibility The SpectraMax i3 Multi-Mode Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. Features. The SpectraMax i2X is used for cell migration, cell. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. , Fisher Scienti fi c, cat. Prepare your i3/i3x instrument for shipment. Simplified workflow: Add cellular imaging assays in a small footprint. Patented user-exchangeable cartridge design expands the system's detection capability making it. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. i3x. Real-time measurement of extracellular oxygen depletion and acidification in cultured cells and microorganisms. Examples: “Press carefully to engage,” “ Do not. Next,. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Myeloperoxidase (MPO) Assay. The plate was shaken and incubated in the dark for 10 minutes. Meanwhile, the samples were detected with a fluorometric reader Spectra Max i3x (MD, America) at 488 nm excitation wavelength and 525 nm emission wavelength. detection cartridge LUM96: 59. These robust readers have been placed in labs from Antarctica to the. 2. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. GFP expression was higher at 21 hours post transfection than at 16 hours (16-hour data not shown). **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. 3, were mixed in a final volume of 100 μL in 10 mM sodium phosphate buffer, pH 7. Benefits • User-upgradeable application modules including cellular imaging • Sensitivity across spectrum with Spectral Fusion™ Illumination • Expanded dynamic range with cooled PMT • Control and analytics provided by SoftMax® Pro Software SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves. 314 low lamp level warning. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Spectramax I3x, supplied by Danaher Inc, used in various techniques. The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube (PMT) for improved detection in extremely low light. The SpectraMax i3x reader grows with you as you continue to advance your research, enabling you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. Molecular Devices are known for both the reliability and the wide uses of their products. In this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. Figure 3. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. The Molecular Devices SpectraMax i3X Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence. Microplate reader assays requiring infrared (IR) or red-shifted. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Relative mitochondrial polarization was expressed as the ratio of emission at 590 nm to that at 530 nm (Shin et al. Here, we demonstrate the benefits of using the SpectraMax ® i3x Multi-Mode Microplate Reader with the SpectraMax ® MiniMax™ 300 Imaging Cytometer in combination with MetaMorph ® Microscopy Automation and Image Analysis Software for performing automated neurite outgrowth imaging and analysis. 2 software (Stat-Ease) to identify factors that have significant effects on cell growth or IgG production, either alone or in combination with. Both Microplate Optimization and Read Height Adjustment were. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. 02, 0. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. A: percent GFP-positive cells calculated using the classification feature in the software. Plate chamber design of the SpectraMax category 1 microplate readers. applications, including dual luciferase and ATP assays. SpectraMax M5. Enzyme activity was presented as absolute activity in a unit of nmol g −1 dry soil h −1. Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. The difference in absorbance observed in the presence and absence of SOD was considered to be the amount of superoxide produced, and results. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. HTRF IP standard curve. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Features. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. Bioz Stars score: 98/100, based on 1 PubMed citations. , 2021). Review date: 19 Mar 2018 | SpectraMax i3x Multi-Mode Microplate Reader with MiniMax Imaging Cytometer The SpectraMax ® line of user-friendly microplate readers provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and moreSimilar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. Kastelic,2 and Jian Gao 1 1Department of Clinical Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China. The calculated LOQ values were 0. AquaMax Microplate Washer. The SpectraMax i3x Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. Louis, MO) and used as received without further purification. Learn how you can perform flash-based applications, such as dual luciferase and ATP assays, with high precision and accuracy using this cartridge. Materials and methodsWe would like to show you a description here but the site won’t allow us. 2. For more information on the SpectraMax i3x Multi-Mode Microplate Reader System, please click here. Quick imaging and analysis of cells gives you a front-row view of the phenotypic changes that accompany. Table 1. In addition, Image J was used to quantify the. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Enzyme Activation Analysis;. *On the SpectraMax i3x reader only. The MiniMax cytometer with SoftMax® Pro Software can be used to image and analyze a variety of cells. 2. Figure 4. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. 0´ 105 dsD NA (p g/ wel ) R F USpectraMax Glo Steady-Luc 工作液都加 入到微孔板后，震荡摇匀并避光放置 10 min。使用 SpectraMax Glo Steady-Luc Reporter 内置的 SpectraMax Glo Steady- Luc Reporter 实验模板设定程序并通过 SpectraMax i3x 读板机检测发光。检测结 束后，模板会根据数据自动生成一条曲 线。SpectraMax MiniMax 300 细胞成像系统 为 SpectraMax i3x 多功能微孔板检测平台而打造！ • • • • 主要特点 系统全面兼容细胞成像 及微孔板检测技术 独特无标记分析技术无需 对细胞染色即可进行细胞 计数和贴壁度的计算 具有多种检测功能可对 细胞活力及毒性进行全. Published Dec 16, 2020 | Updated Oct 10, 2023. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. SpectraMax i3x 多功能微孔板读板机 SpectraMax Paradigm 多功能微孔板读板机 SpectraMax M5e 多功能微孔板读板机 扫一扫关注我 们的官方微信 Molecular Devices 大中华区 全国咨询服务热线: 400-820-3586 Email: info. The CellTiter-Glo assay from Promega uses luciferase. The TUNE detection cartridge uses a breakthrough monochromator design that enables researchers to easily optimize their. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Contact Us Phone: +1. 99（图一）。The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. See SpectraMax® Microplate Reader User Manual Download Page. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. i3. Promega. 3) At the bottom of the strip, click on "Show. This assay uses two fluorescent dyes, calcein AM (cal AM) and ethidium. ) wide and 65 cm (25. It allows the user to determine the total concentration of protein in a solution. The SpectraMax i3x reader provides the. Description 1. Turbidity measurements were performed with a Spectra Max® i3x microplate reader to study the effect of oxidation on the kinetics of sFN formation. The SpectraMax i3x reader provides theThe SpectraMax® i3x Multi-Mode Microplate Reader with the SpectraMax® MiniMax™ 300 Imaging Cytometer can be used to image slides using a specialized slide adapter. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. We would like to show you a description here but the site won’t allow us. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a. Multimode Readers Comparison Chart. Shake intensity. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. 9 x 10-15. Measured using the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. The SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. Superior flexibility The SpectraMax i3 Multi-Mode Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. The chamber. Quantification of NETs was performed with the SpectraMax i3x microplate-reader (Molecular Devices, United Kingdom). Bound crystal violet was dissolved with 10% acetic acid and the optical density (OD) at 570 nm was determined using a Spectra Max i3X spectrophotometer (Molecular Devices). 305 reference level saturation. Absorbance was measured on the SpectraMax i3x Multi-Mode Platform at 475 nm and 660 nm after 1-4 hours of incubation. The SpectraMax i3 is specifically used in this lab for BCA assays and Elisa Assays. This application note describes the use of the SPECTRAmax ® GEMINI XS from Molecular Devices to study the performance of the LIVE/DEAD ® Viability/Cytoxicity Assay Kit for animal cells. GFP expression imaged on the cytometer. Synthesis of ZM-1NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. SpectraMax Microplate Readers. Patented user-exchangeable cartridge design expands the system's detection capability making it. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. 0001 0. 3. Fluorescence Reader. 4 / 5. The SpectraMax i2X is used for cell migration, cell. The plate was shaken and incubated in the dark for 10 minutes. SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. ATP (“flash” luminescence using . The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring SpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. com. Remove the transport bracket and store the retaining screws in the bag that held the 3. Spectranet customer portalWe would like to show you a description here but the site won’t allow us. Example: [Drawer]. Also refer to this Multimode Reader Comparison Chart. The SpectraMax i3 also allows the user to identify antibodies during Elisa Assays. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. 0´ 104 4. The SpectraMax ® i3x reader provides the. *On the SpectraMax i3x reader only. Time-resolved fluorescence was measured on the SpectraMax Multi-Mode Microplate Readers (see Table 2 for instrument settings). The SpectraMax® i3 Platform's base system features an integrated optical system enabling top and bottom reads for 6-, 384- well microplates and launches with three broad detection modes: luminescence, absorbance, and fluorescence. Note: Additional readers with similar performance are listed. Structured Review. See Unpacking the SpectraMax Paradigm Instrument on page 1. pathways and protein activation and expression in one system. Table 1. Unpacking and setting up the instrument includes the following procedures: • Unpacking the instrument and saving the original packaging. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. More Product Information. 6 in. Spectramax I3x, supplied by Danaher Inc, used in various techniques. As shown in the results above, the DELFIA cell proliferation assay combined with the SpectraMax i3 reader gives researchers a very sensitive test for measuring cell proliferation. It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. Page 9 Installing and Setting Up the SpectraMax MiniMax Imaging Cytometer Attaching the Imaging Cytometer to the Microplate Reader Attaching the SpectraMax MiniMax Imaging Cytometer to the SpectraMax i3x Instrument requires a work area that is approximately 130 cm (51. Products : SoftMax Pro Software, SoftMax Pro GxP Software, CellReporterXpress software, General, Screenworks, pCLAMP/AxoScope, GenePix Pro, MetaXpress software. Find regional support. MD Neurotransmitter Transporter Assay. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Prepare your i3/i3x instrument for shipment. For each assay kit, a preconfigured protocol is included in SoftMax Pro Software to facilitate the acquisition and analysis of data for a simplified workflow. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the. Analysis of HTRF assays involves Cisbio’s patented ratiometric reduction method based on the two emission wavelengths detected. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologySpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. In this product video, see how the SpectraMax M5 quickly reads samples. We would like to show you a description here but the site won’t allow us.